Derek Parks

CREARE Open Water Logger

Wireless Open Water Logger for Ocean Sampling

The Wireless Open Water Logger (WOWL) Project is a NOAA-funded effort by Creare LLC, in collaboration with colleagues at the University of Maine’s Darling Marine Center. The goal is to enable large-scale opportunistic ocean sampling by developing a low-cost, reliable, and open-source ocean temperature and depth logger. Changes in ocean temperature have a profound impact on the productivity of fisheries and aquaculture. Sampling offshore ocean temperatures, particularly at depth, is challenging and expensive. Opportunistic sampling from fishing gear can address this sampling need. Commercial fishermen are more and more aware of the correlations between water temperature and harvest. Existing programs, such as Environmental Monitoring on Lobster Traps (EMOLT, a collaboration between NOAA and the Gulf of Maine Lobster Foundation), have capitalized on this on a small (but ever increasing) basis. These data are already contributing to our understanding of links between temperature and catch. Expanding these programs is hindered by the high cost of sensor nodes and the need for integrating data-transmission hardware with the host ship. A simpler and less expensive approach is needed. The WOWL is designed to inexpensive, rugged, and easy to use. The WOWL integrates with users’ smartphones (via Bluetooth Low Energy and a free application), eliminating the need for dedicated hardware to retrieve and upload data. No ship integration is necessary. Our vision for the future is that data are geo-tagged by the smartphone and then uploaded to an existing, cloud-based, and publicly accessible data repository. The data can be accessed via standard, open data formats (e.g., CSV text files) from both the mobile application and the cloud repository. Data will be visualized as soon as it is uploaded via WeatherCitizen or via open online visualization toolkits. Resources: Project Website GitHub Repository For additional information please contact the Creare team at wowl@creare.com. Federal Procurement – Sole Source Information The right to receive sole-source awards is a real benefit of the SBIR Program both to the government and to the participating companies. The SBA SBIR/STTR Policy Directive dictates the justification that an agency can and must use to justify the sole-source award. Predictably, that justification reads that the new award must derive from, extend, or complete prior SBIR effort and be funded with non- SBIR funds.

Figure 1: Rendering of the air-deployed Black Swift S0 for use in hurricane research.

Black Swift Technologies – SØ™ UAS Platform

Black Swift Technologies works through NOAA and Air Force SBIR to Develop Multi-Mission UAS Capabilities The Black Swift SØ™ UAS is an intelligent unmanned aircraft capable of completely automatic flights at altitudes up to 15,000 feet AGL. Its unique tail design enables deep-stall landing with near vertical descent and 10-foot landing accuracy. The rapid ascent and descent allow the SØ to quickly and accurately capture 3-dimensional wind profiles at various levels. With a flight duration of up to 90 minutes, the Black Swift SØ has the endurance and comprehensive sensor suite necessary to capture a profile of upper air parameters including air temperature, wind speed and direction, dewpoint, and atmospheric pressure–quickly and efficiently. Manufactured entirely in the USA, the SØ was engineered from inception for flight s in extreme atmospheric conditions, including hurricane deployments. Its advanced avionics enables fully autonomous operation with minimal training. Designed for rapid atmospheric profiling, the SØ’s automated sampling patterns and scripting allow for “launch-and-forget” operations.  Learn More –> For procurement inquiries, contact sales@bst.aero. Federal Procurement – Sole Source Information The right to receive sole-source awards is a real benefit of the SBIR Program both to the government and to the participating companies. The SBA SBIR/STTR Policy Directive dictates the justification that an agency can and must use to justify the sole-source award. Predictably, that justification reads that the new award must derive from, extend, or complete prior SBIR effort and be funded with non- SBIR funds.

The World Climate Service provides meteorologists and other climate-sensitive professionals with critical long-range forecast information to support climate risk-related decision making.

World Climate Service – Long Lead Forecasting Tools

The World Climate Service provides meteorologists and other climate-sensitive professionals with critical long-range forecast information to support climate risk-related decision making. As forecasting skill improves at all lead times, new opportunities emerge to provide innovative and valuable services. Founded in 2009, the World Climate Service is positioned to be a leader in the exciting developments and upcoming growth of long-range weather and climate forecasting. More Information –> Federal Procurement – Sole Source Information The right to receive sole-source awards is a real benefit of the SBIR Program both to the government and to the participating companies. The SBA SBIR/STTR Policy Directive dictates the justification that an agency can and must use to justify the sole-source award. Predictably, that justification reads that the new award must derive from, extend, or complete prior SBIR effort and be funded with non- SBIR funds.

Nucleic Sensing Systems eDNA Tracker shown at water's edge

Autonomous, Real-Time, Field-Deployable Genetic Detection Technology

The Tracker is the world’s only field-deployable, automatic, web-connected monitoring tool, able to report real-time data on environmental DNA (eDNA). By taking the human out of the loop, we connect autonomous high-frequency sampling with geospatial data to better monitor biological activity. Tracker Capabilities Continuous-flow operation giving real-time results Operating with commercially available liquid reagents that require no refrigeration which eases logistical concerns No concentration, separation, or purification of samples required Simultaneous multi-species detection enables geospatial and temporal data for mapping and optimizing a management response Autonomous, GPS integration, Cloud-connected software interface with data & alerts Digital genetic verification via PCR with general specificity Federal Procurement – Sole Source Information The right to receive sole-source awards is a real benefit of the SBIR Program both to the government and to the participating companies. The SBA SBIR/STTR Policy Directive dictates the justification that an agency can and must use to justify the sole-source award. Predictably, that justification reads that the new award must derive from, extend, or complete prior SBIR effort and be funded with non- SBIR funds. For inquiries about this technology, please contact Nucleic Sensing Systems directly. 1-Page Fact Sheet

Giner Harmful Algal Bloom Detection Instrument next to aerial view of a harmful algal bloom on the water.

Low Cost Instrument for Detection of Toxins in Seawater During Harmful Algal Blooms

Technology Overview Giner, Inc. (Giner) has developed a low-cost, portable instrument for detection of toxins found in seawater during harmful algal blooms (HABs). During these HAB events the rapid growth of Karenia brevis algae leads to a significant increase in local concentrations of a marine neurotoxin, brevetoxin (BTX). High density blooms of K. brevis, “red tides”, are endemic to the Gulf of Mexico and frequently occur on a nearly annual basis. Giner’s field-ready toxin analyzer, developed with NOAA SBIR Phase II funding, uses aptamer modified screen printed electrodes (SPEs) to bind with brevetoxin present in ocean water. This is followed by a rapid electrochemical analysis step to elucidate toxin concentrations. The resulting assay is portable and more cost efficient than traditional methods but has a similar limit of detection, making it possible for use as a red tide early warning tool. This novel assay has been incorporated into a custom prototype handheld device that performs the electrochemical detection step and data recording either locally or by Bluetooth using a nearby tablet or laptop computer. The complete instrument can be easily carried and used along the Florida coastline to better detect and monitor the severity of HAB events. Advantages Traditional benchtop instruments (i.e. HPLC-MS) used to measure brevetoxin are bulky, expensive, and they require skilled technicians to operate. Sample-to-result turnaround time is, at best, on the order of hours but typically requires several days. The health and economic effects of a red tide on nearby communities are exacerbated by this delay. Additionally, samples need to be collected, transported, and sometimes stored at the testing facility prior to analysis, creating several logistical hurdles for those performing the analysis. Conversely, ELISA or other antibody based immunoassay techniques are more accessible while performing field work, but they lack the sensitivity and selectivity of traditional chromatography techniques. These assays also necessitate a cocktail of complex reagents that do not store or travel well. Giner’s electrochemical toxin assay and the analyzer instrument would provide HAB researchers and public health officials with several advantages over the current state of the art toxin monitoring. The aptamer functionalized SPEs are inexpensive, easy to fabricate and can be stored for months before use. Testing is rapid (<30 min sample-to-result) and inexpensive (<$15/sample). Lastly, the parts-per-billion level limit of detection can alert officials to a red tide event far before the current visual methodologies of beach observation and satellite imagery. Partners Florida Map with MOTE Beach Conditions Reporting Sites (Credit: MOTE Marine Lab) Through the NOAA Phase II funding, Giner was able to partner with Mote Marine Laboratory (Mote), based in Sarasota, FL, to guide development of the sensor to better fit the needs of potential end users. Giner worked closely with Dr. Richard Pierce (Senior Scientist for Ecotoxicology at Mote) who provided valuable insight on red tide ecology as well as the necessary K. brevis lab work.  This collaboration has subsequently led to several other HAB related partnerships, including adaptation of Giner’s technology to detect aerosolized brevetoxin found in ocean spray to monitor respiratory health concerns for communities adjacent to Florida’s coastal regions. By leveraging Mote’s Beach Conditions Reporting System (BCRS), Giner’s rapid aerosol toxin data would feed into the daily reports of red tide conditions (e.g. respiratory irritation, dead fish, and discolored waters). Giner’s goal is to distribute a handheld portable instrument to trained beach sentinels (lifeguards, government agents, and vetted volunteers) to improve airborne detection and data resolution. The BCRS has become a staple for residents and visitors, acquiring over 1 million users and over 4 million page views to the website alone. Data from this effort is also automatically sent to GCOOS, NOAA, and FWC. Federal Procurement Information The right to receive sole-source awards is a real benefit of the SBIR Program both to the government and to the participating companies. The SBA SBIR/STTR Policy Directive dictates the justification that an agency can and must use to justify the sole-source award. Predictably, that justification reads that the new award must derive from, extend, or complete prior SBIR effort and be funded with non- SBIR funds. For inquiries about this technology, please contact Giner, Inc. directly.